OBESITY-Environmental Factors and/or genetic Influence Genomic Structure, Mutational Analysis andPromoter Function of The Human Uncoupling Protein-2/-3 (hUCP2/hUCP3) Genes
نویسنده
چکیده
4 Rection). Polymorphism analysis by Genotyping. Determination of functional properties of the 5’flanking and the promoter region of these two genes: elucidation the 5’ Flanking Region of the hUCP2 gene by PCR-screening a human genomic library. Genome Walking of 5’Flanking Region of human UCP3 Gene. Promoter analysis human UCP –2 /-3 utilizing pCAT-3 reporter gene system, transient transfection, CAT ELISA and protein determination
منابع مشابه
Expression of human uncoupling protein-3 in Drosophila insulin-producing cells increases insulin-like peptide (DILP) levels and shortens lifespan
Uncoupling proteins (UCPs) can dissipate mitochondrial protonmotive force by increasing the proton conductance of the inner membrane and through this effect could decrease ROS production, ameliorate oxidative stress and extend lifespan. We investigated whether ubiquitous, pan-neuronal or neurosecretory cell-specific expression of human UCP3 (hUCP3) in adult Drosophila melanogaster affected life...
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